Phytochemistry & Biodiscovery

Major Analytical Instruments:

For metabolic profiling/analysis/dereplication of multicomponent mixtures from different matrices

  • UPC2: Waters Acquity UPC2 system with PDA and evaporative light scattering detector (ELSD), and QDa mass detector
  • UPLC systems with different selective (such as UV/Vis, diode array and QDa mass detectors) and universal detectors, such as evaporative light scattering (ELSD) and charged aerosol detectors (CAD)
  • UPLC - triple quadrupole mass spectrometer with ESI and APCI ion source for the sensitive quantification in complex matrices
  • UPLC - ion trap mass spectrometer with ESI and APCI ion source for metabolite profiling and dereplication
  • Capillary gas chromatograph combined with a single quadrupole spectrometer (GC-MS) with EI- and CI-ionization for the identification of volatile compounds
  • Different HPLC systems with UV/Vis, DAD and/or ELSD for routine analysis
  • Gas chromatography instruments with flame ionization detectors (FID)
  • High-performance TLC system with sample application unit, automatic chromatographic chamber, TLC visualizer and evaluation unit

For structural characterization of novel natural products

  • A high-resolution quadrupole time-of-flight mass spectrometer (QTOF-MS) with ESI and APCI ion source (with UPLC system)*
  • Dedicated NMR spectrometers (400 MHz and 500 MHz) with autosampler and cryoprobe*
  • UV/VIS spectrophotometers
  • Attenuated total reflection (ATR) - Fourier transform infrared (FTIR) spectrophotometer

* shared use with or access via the Department of Pharmaceutical Chemistry

For fractionation and targeted isolation of metabolites

  • Preparative supercritical fluid chromatograph (SFC) with parallel detection units (PDA, ELSD, QDa) and fraction collector for isolation with minimial organic solvent consumption
  • Preparative HPLC systems with autosampler, DAD and fraction collector
  • High performance counter-current chromatograph (HPCCC)
  • Ultra-performance flash chromatograph combined with PDA and ELSD

For sample preparation

  • Accelerated solvent extraction (ASE) system
  • Centrifugal evaporator
  • Benchtop manifold freeze-drier