Phytochemistry & Biodiscovery

Major Analytical Instruments:

For metabolic profiling/analysis/dereplication of multicomponent mixtures from different matrices

  • UHPSFC: Waters Acquity UPC2 system with PDA, evaporative light scattering detector (ELSD), and quadrupole Dalton (QDa) mass detector
  • UPLC systems with different selective (such as UV/Vis, diode array and mass detectors) and universal detectors, such as ELSD and charged aerosol detectors (CAD)
  • UPLC-SCIEX Triple Quad 4500 System mass spectrometer with ESI and APCI ion source for the sensitive quantitation in complex matrices
  • UPLC-Thermo LTQ XL™ Linear Ion Trap Mass Spectrometer with ESI and APCI ion source for metabolite profiling and dereplication
  • Capillary gas chromatograph combined with a single quadrupole spectrometer (GC-MS) with EI- and CI-ionization for the identification of volatile compounds
  • Different HPLC systems with UV/Vis, DAD and/or ELSD for routine analysis
  • Gas chromatography instruments with flame ionization detectors (FID)
  • High-performance TLC instrumentation with sample application unit, automatic chromatographic chamber, TLC visualizer and evaluation unit

For structural characterization of novel natural products

  • UPLC-X500 QTOF Mass Spectrometer Systems with ESI and APCI ion source
  • Bruker high resolution NMR spectrometers (400 MHz and 500 MHz) with autosampler for 60 samples and Prodigy CryoProbe (TCI) for enhanced sensitivity*
  • UV/VIS spectrophotometers
  • Attenuated total reflection (ATR) - Fourier transform infrared (FTIR) spectrophotometer

*) shared use with or access via the Division of Pharmaceutical Chemistry

For fractionation and targeted isolation of metabolites

  • Preparative supercritical fluid chromatograph (Waters SFC Prep-15) with parallel detection units (PDA, ELSD, QDa) and fraction collector for isolation with a minimum of organic solvent consumption
  • Preparative HPLC systems with autosampler, DAD and fraction collector
  • High performance counter-current chromatograph (HPCCC)
  • Ultra-performance flash chromatograph combined with PDA and ELSD

For sample preparation

  • Accelerated solvent extraction (ASE) instrument:
    Thermo Fisher Scientific Dionex ASE 350
  • Supercritical fluid extraction (SFE) instrument: Waters MV-10
  • Centrifugal evaporator
  • Benchtop manifold freeze-drier