Phytochemistry & Biodiscovery

Identity and purity of the starting material are prerequisites for safety and efficacy of herbal medicinal products. For unambiguous identification and quality assessment of herbal drugs and their preparations we use robust, quick and reliable methods like HPTLC, microscopy, UV/VIS-spectrophotometry, GC, UPLC and FTIR. Sophisticated combinations of high-performance separation techniques with specific high-end detectors (e.g. GC-MS, UPLC-DAD-MSⁿ, UPC²-PDA-QDa, UPLC-MS/MS) allow for the identification and exact quantification of very small amounts of compounds, even present in complex matrices or mixtures like plant extracts, tissues or body fluids.

Selected Papers:

Goels T, Eichenauer E, Langeder J, Aichner GF, Mauser G, Amtmann L, Grienke U, Glasl S. Ultra High-Performance Supercritical Fluid Chromatography for the Quantitation of Diterpene Resin Acids in Norway Spruce Samples. Front Pharmacol. 2022; 13: 906411. doi: 10.3389/fphar.2022.906411.

Langeder J, Grienke U. A supercritical fluid workflow for the quality assessment of herbal drugs and commercial preparations from Rhodiola rosea. Phytochemical Analysis 2021; 32: 982-991. 10.1002/pca.3040

Langeder J, Grienke U, Döring K, Jafari M, Ehrhardt C, Schmidtke M, Rollinger JM. High-performance Countercurrent Chromatography to Access Rhodiola rosea Influenza Virus Inhibiting Constituents. Planta Med. 2021 Aug; 87(10-11):818-826.10.1055/a-1228-8473

Tobudic S, Prager I, Kussmann M, Obermueller M, Ursli M, Zeitlinger M, Wiesholzer M, Burgmann H, Poeppl W, Reznicek G. Compatibility of aztreonam in four commercial peritoneal dialysis fluids. Scientific Reports 2020; 10: 1788. 10.1038/s41598-020-58391-y

Schretter C, Langeder J, Freisinger V, Rollinger JM, Grienke U. Quantitative analysis of prenylated constituents in commercial hops samples using ultrahigh-performance supercritical fluid chromatography. Planta Med. 2020; 86: 1140-1147. 10.1055/a-1130-0590

Schoeny H, Rampler E, Hermann G, Grienke U, Rollinger JM, Koellensperger G. Preparative supercritical fluid chromatography for lipid class fractionation-a novel strategy in high-resolution mass spectrometry based lipidomics. Analytical and Bioanalytical Chemistry 2020; 412: 2365-2374. 10.1007/s00216-020-02463-5